Measure on-
and off-target
engagement
across the RBPome
RBP Selectivity Profiling (RBP-SP™) is used when prioritized compounds require confirmation of cellular target engagement and early assessment of off-target liabilities. By directly measuring compound-induced changes in endogenous RNA–protein interactions across the RNA-binding proteome, RBP-SP™ provides an unbiased, in-cell view of engagement beyond the intended target.
This service applies mass-spectrometry–based profiling to quantify RNA–protein interaction–coupled engagement across hundreds of RBPs in parallel. Because measurements are performed in intact cells without tags, reporters, or overexpression systems, RBP-SP™ preserves native biology and avoids assumptions based on protein stability or proxy readouts.
As a result, RBP-SP™ is well-suited for in-cell hit confirmation and early selectivity de-risking. The resulting RBPome-scale engagement and selectivity profiles enable confident assessment of whether observed activity is supported by direct cellular engagement of the intended RNA-binding protein, while identifying secondary and off-target perturbations before compounds advance.
A QUICK LOOK AT HOW IT WORKS
The path of an RBP Selectivity Profiling Project
Project Start
Plan your project with our dedicated scientists. Align on study design and timelines.
Material Submission
Submit your compounds and/or cell lines to our team, and we will work with you to answer any questions you may have regarding handling, storage, or preparation.
Work Begins
We use optimized workflows and state-of-the-art equipment to perform the project. Once data is generated, we apply standardized processing and analysis pipelines to ensure accurate, consistent, and high-quality results.
Results with Expert Interpretation
When the analysis is complete, we share the results with you and guide you through the key findings, helping you understand the implications for your program.
Discover how our platform can tackle your drug discovery challenges and unlock new therapeutic opportunities. Get in touch or book a free scoping call with one of our experts.