Advance
the Right Compounds
RBP Target Engagement (RBP-TE™) is used when mechanistic hypotheses or RNA-binding protein targets are already defined. By directly measuring compound-induced changes in endogenous RNA–protein interactions in cells, the assay provides focused, quantitative evidence of cellular target engagement.
This service lets you establish quantitative RBP-TE™ assays in your selected cellular matrix, enabling RNA–protein interaction–coupled target engagement measurements under physiologically relevant conditions, without tags, overexpression, or reporter systems. The assay can be used to generate IC₅₀ values based on in-cell target engagements, supporting hit-to-lead decisions by differentiating on-target engagement from off-target effects and identifying cases where downstream phenotypic responses are not supported by direct target engagement.
As a result, RBP-TE™ is well-suited for validating mechanistic hypotheses and confirming that compounds engage the intended RNA-binding protein(s) in their native cellular context. The resulting cellular target engagement IC₅₀ values and dose–response diagnostics support confident hit confirmation, engagement-informed hit-to-lead progression, and early lead optimization by enabling quantitative SAR interpretation, compound ranking, and lead series prioritization.
A QUICK LOOK AT HOW IT WORKS
The path of an RBP Target Engagement Project
Project Start
Our scientists collaborate with you to define assay parameters, acceptance criteria, and study endpoints tailored to your specific target and compound series.
Material Submission
Submit your compounds and/or cell lines to our team, and we will work with you to answer any questions you may have regarding handling, storage, or preparation.
Work Begins
We develop and execute the validated assay using well-characterized reagents and rigorous QC standards. We stay in touch with you throughout the study and provide updates on its progress.
Results with Expert Interpretation
When analysis is complete, we share comprehensive results and guide you through the key findings, discussing how they connect to your research goals, scientist-to-scientist.
Discover how our platform can tackle your drug discovery challenges and unlock new therapeutic opportunities. Get in touch or book a free scoping call with one of our experts.
A RBP TARGET ENGAGEMENT (RBP-TE™) APPLICATIONS
Hit Confirmation
Confirm cellular target engagement of prioritized hits in live cells to triage compounds that directly engage the intended RNA-binding protein from those lacking measurable engagement.
Compound Prioritization
Quantify concentration-dependent cellular target engagement across compounds to enable engagement-based ranking, inform SAR decisions, and support data-driven compound prioritization.