Rank Compounds Based On Quantitative Cellular Target Engagement
Label-free target engagement measurements in a physiologically relevant cellular matrix provide rigorous, quantitative evidence to support compound prioritization. Interpreted alongside downstream functional readouts, they support SAR interpretation, compound ranking, and confident lead series prioritization.
Assay conditions established during earlier stages are maintained to enable meaningful comparison across compounds. Replication strategies and normalization schemes are defined to generate reliable data for decision-making and facilitate consistent comparisons across studies. Curve behavior, model fit, and pre-specified quality thresholds are evaluated to ensure that engagement measurements support confident and consistent SAR interpretation.
Throughout this phase, assay performance and data quality are monitored to ensure engagement measurements are suitable for decision-making. Cellular target engagement measurements generated by RBP-TE™ assays support quantitative SAR interpretation, compound ranking, and confident lead series prioritization, ensuring that compound progression decisions are not solely reliant on proxy measurements or downstream functional outcomes.
Prioritize Compounds with Mechanistic Clarity
Incorporation of the RBP Target Engagement (RBP-TE™) assay for compound prioritization ensures that differences in downstream functional outcomes align with differences in target engagement in the intended, physiologically relevant cellular matrix, accelerating SAR progression and supporting the identification of high-quality lead compounds.
Compound prioritization is enabled by our RBP Target Engagement (RBP-TE™) service.
RBP Target Engagement
(RBP-TE™)
Focused, quantitative target engagement
Label-free target engagement assays that quantify engagement IC₅₀ values and time-dependent engagement kinetics for nominated RNA-binding protein targets to support medicinal chemistry optimization.