Select hits that bind in cells from day one 

Primary screening identifies starting points for new therapeutic programs that can be developed into compounds showing meaningful activity in biologically relevant contexts. Compound libraries are screened using biochemical or cell-based methods against defined targets, with early hits supporting subsequent optimization and development.

Decisions around biological relevance, assay technology, throughput, and library selection are addressed early, as these factors directly influence screening efficiency, reproducibility, and hit quality. Assays are optimized and validated with clear performance criteria to ensure robustness across screening campaigns. For high-throughput screening, practical considerations such as automation, liquid handling, and assay controls are incorporated from the outset. Data analysis strategies are defined to identify true hits while controlling false positives and minimizing bias. Pilot screens assess signal window, Z′-factor, and assay variability before full-scale screening.